Journal: EMBO molecular medicine

Article Title: Mitochondrial MsrB2 serves as a switch and transducer for mitophagy.

doi: 10.15252/emmm.201910409

Figure Lengend Snippet: Figure 1. Identification of MsrB2 as LC3 interaction protein in human platelets.

Article Snippet: Healthy/DM platelet lysates and cell lysates (after transient transfection) were mixed with the specific target antibody (1 lg of LC3 antirabbit antibody (Abcam) and 2 lg of Parkin anti-goat antibody (Abcam) or 1.5 lg of Parkin anti-rabbit antibody (Abcam) for Parkin IP; 1.5 lg of MsrB2 anti-rabbit antibody (laboratory made) for MsrB2 IP; and 1 lg of GFP anti-rabbit antibody (Abcam, USA) or GFP-Trap bead (Chromotek, USA), and the same species IgG control with HC) and incubated overnight at 4°C.

Techniques:

Journal: EMBO molecular medicine

Article Title: Mitochondrial MsrB2 serves as a switch and transducer for mitophagy.

doi: 10.15252/emmm.201910409

Figure Lengend Snippet: Figure 2. MsrB2 is needed for mitophagy.

Article Snippet: Healthy/DM platelet lysates and cell lysates (after transient transfection) were mixed with the specific target antibody (1 lg of LC3 antirabbit antibody (Abcam) and 2 lg of Parkin anti-goat antibody (Abcam) or 1.5 lg of Parkin anti-rabbit antibody (Abcam) for Parkin IP; 1.5 lg of MsrB2 anti-rabbit antibody (laboratory made) for MsrB2 IP; and 1 lg of GFP anti-rabbit antibody (Abcam, USA) or GFP-Trap bead (Chromotek, USA), and the same species IgG control with HC) and incubated overnight at 4°C.

Techniques:

Journal: EMBO molecular medicine

Article Title: Mitochondrial MsrB2 serves as a switch and transducer for mitophagy.

doi: 10.15252/emmm.201910409

Figure Lengend Snippet: Figure 5. Methionine sulfoxidation (MetO) and methionine sulfide reductase B2 (MsrB2) are increased, along with Parkin, in DM platelets.

Article Snippet: Healthy/DM platelet lysates and cell lysates (after transient transfection) were mixed with the specific target antibody (1 lg of LC3 antirabbit antibody (Abcam) and 2 lg of Parkin anti-goat antibody (Abcam) or 1.5 lg of Parkin anti-rabbit antibody (Abcam) for Parkin IP; 1.5 lg of MsrB2 anti-rabbit antibody (laboratory made) for MsrB2 IP; and 1 lg of GFP anti-rabbit antibody (Abcam, USA) or GFP-Trap bead (Chromotek, USA), and the same species IgG control with HC) and incubated overnight at 4°C.

Techniques:

Journal: EMBO molecular medicine

Article Title: Mitochondrial MsrB2 serves as a switch and transducer for mitophagy.

doi: 10.15252/emmm.201910409

Figure Lengend Snippet: Figure 6. MsrB2 is ubiquitinated by Parkin.

Article Snippet: Healthy/DM platelet lysates and cell lysates (after transient transfection) were mixed with the specific target antibody (1 lg of LC3 antirabbit antibody (Abcam) and 2 lg of Parkin anti-goat antibody (Abcam) or 1.5 lg of Parkin anti-rabbit antibody (Abcam) for Parkin IP; 1.5 lg of MsrB2 anti-rabbit antibody (laboratory made) for MsrB2 IP; and 1 lg of GFP anti-rabbit antibody (Abcam, USA) or GFP-Trap bead (Chromotek, USA), and the same species IgG control with HC) and incubated overnight at 4°C.

Techniques:

Journal: EMBO molecular medicine

Article Title: Mitochondrial MsrB2 serves as a switch and transducer for mitophagy.

doi: 10.15252/emmm.201910409

Figure Lengend Snippet: Figure 7. MsrB2 is released by mitochondrial rupture.

Article Snippet: Healthy/DM platelet lysates and cell lysates (after transient transfection) were mixed with the specific target antibody (1 lg of LC3 antirabbit antibody (Abcam) and 2 lg of Parkin anti-goat antibody (Abcam) or 1.5 lg of Parkin anti-rabbit antibody (Abcam) for Parkin IP; 1.5 lg of MsrB2 anti-rabbit antibody (laboratory made) for MsrB2 IP; and 1 lg of GFP anti-rabbit antibody (Abcam, USA) or GFP-Trap bead (Chromotek, USA), and the same species IgG control with HC) and incubated overnight at 4°C.

Techniques: